The essential oils of the aerial parts of Hypericum perfoliatum L., collected in two different locations in Greece, were obtained by hydrodistillation and analysed by GC-MS. Fifty constituents were identified. The monoterpene hydrocarbons had the highest contribution. The major constituents in sample A were˛-pinene (48.6%) n-nonane (8.5%) and υ-cadinene (4.6%), while those of sample B, were˛-pinene (34.2%)ˇ-pinene (9.2%) and υ-cadinene (8.1%).

From the aerial parts of Hypericum triquetrifolium Turra (Guttiferae) 3-8"-biapigenin, quercetin, rutin and chlorogenic acid were isolated. Their structures were elucidated by spectral techniques. Furthermore, the phenolic compounds were tested for antioxidant activity, using an in vitro method based on sodium arachidonoate, bleomycin and thiobarbituric acid with alpha-tocopherol as the reference solution.

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