, in charge of the Physics Division. He was formerly a physicist in the Government Erskine Hospital, Madurai. He holds an M.A. degree in Physics with electronics as special subject and an M.Sc. degree in Spectroscopy, the Annamalai University.
Spectrofluorometric quantitation of a lignan lactone, diphyllin, and its glycosides cleistanthin A and cleistanthin B, the active principles of the poisonous plant Cleistanthus collinus, is reported for the first time. Thin layer chromatographic resolution of the components precedes the fluorometric measurements. The method is highly reproducible and sensitive to 0.1 microgram/mL for diphyllin and to 1 microgram/mL for cleistanthins A and B in ethanol. The technique is employed for the assay of several biospecimens of forensic and clinical importance.
Blood samples from 240 unrelated healthy Tamil-speaking South Indian Hindus residing in Madras (capital city of Tamil Nadu, India) were screened for HLA-A and -B antigen profiles. Antigen, gene and haplotype frequencies were calculated and compared with the literature. Tamil Hindus lack A31, A32, Aw33, B16, B21 and Bw41. However, except for minor differences (low occurrence of Aw19 antigen), the South Indians show similarity to North Indian and other Indian groups. The data confirm once more that the haplotype A1-B17 is characteristic of Indians.
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