Introduction:The bacterial resistance to antibiotics is becoming a serious concern in developing countries due to precarious hygiene conditions, inappropriate prescription as well as self-medication and free sale of antibiotics.The general objective of this study is to assess the prevalence of strains producing ESBL in the BGN isolated in the CHUP-CDG laboratory.Methods: It is a prospective cross-sectional descriptive study conducted from 1 May to 12 August 2014.The study was conducted in the Charles De Gaulle paediatric University Hospital (Ouagadougou) and the Arnaud de Villeneuve CHRU (Montpellier).Results: Out of the 889 pathological substances analysed, 175 germs have been isolated among which 110 BGN which equal 62.8%. 48.2% of our BGN are ESBL +.Among the 110 BGN we isolated 101 EB and 9 other BGN. No ESBL was found in the other BGN and 52.5% of EB were ESBL +. As for the ESBL Phenotype, according to the species/gender 60.4% of ESBL were E coli and 32% were K pneumoniae.