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Objective To compare and quantify, in a morphological (91), 81 (20) and 74 (38) nerves/mm2 in the three defined areas, respectively. In the neuropathic tissues study, the changes that occur in the connective tissue elements (elastin and collagen), muscle fibre diameters the nerve profile densities were 672 (249), 57 (23) and 37 (28) nerves/mm2, respectively. Fibre diameter, and nerve densities between normal, idiopathic and neuropathic bladders.elastin and collagen content and nerve density were measured in normal and unstable bladder tissue using Materials and methods Bladder tissue was obtained from 27 patients undergoing cystectomy for carcithese three defined areas. The mean (sem) fibre diameter was 6.81 (0.52) in normal bladder; in idiopathic noma, from 12 with idiopathic instability and from seven neuropathic patients who were undergoing ileobladder tissue the fibre diameters in the three areas were 6.72 (0.62), 7.06 (0.62) and 7.34 (1.15) mm, cystoplasty. A combination of histochemical and immunohistochemical techniques were used to detect respectively, and in neuropathic bladders were 6.75 (0.62), 8.24 (0.62) and 9.35 (0.62) mm, respectively. detrusor muscle, connective tissue and nerve profiles in the bladder tissue.The relative areas of elastin were 0.79 (0.70), 0.56 (0.45) and 18.3 (4.1)% for the control, normal and Results In both idiopathic and neuropathic bladder tissue the structural changes were highly punctate. From aCected areas of the neuropathic bladders, respectively, and the relative areas of collagen were 3.5 (1.3), 6.15

The nucleotide sequence of the M2 gene of pneumonia virus of mice (PVM) was determined. The sequence showed that the gene encoded a protein of 176 amino acids with a predicted molecular mass of 20 165 Da from a major ORF, which is smaller than the equivalent proteins encoded by human, bovine and ovine respiratory syncytial (RS) viruses. The PVM M2 protein is conserved, having 41 % similarity to the equivalent human RS virus protein. In common with the M2 genes of the RS viruses and avian pneumovirus (APV), the PVM mRNA also contained a second ORF (ORF2) that partially overlaps the first ORF and which is capable of encoding a 98 residue polypeptide. No significant sequence identity could be detected between the putative M2 ORF2 proteins of PVM, APV and the RS viruses. The expression of the M2 ORF2 proteins of the pneumoviruses was investigated by using monospecific antisera raised against GST fusion proteins. Western blot analysis demonstrated the presence of polypeptides encoded by M2 ORF2 of PVM and RS virus corresponding with those predicted by in vitro translation studies, but this was not the case for APV. The PVM polypeptide was present as three distinct products in vivo. The PVM and RS virus polypeptides were also detected in cells by immunofluorescence, which showed that both were present in the cytoplasm with a degree of localization in inclusion bodies. No APV M2 ORF2 protein could be detected in vivo. The RS virus M2 ORF2 polypeptide was shown to accumulate during infection and the potential implications of this are discussed.