ABSTRACT. We examined the protective effects of ultrafiltered XinMaiJia (XMJ) extract in a hydrogen peroxide (H 2 O 2 )-induced injury model in human umbilical vein endothelial cells (HUVECs) and determined the corresponding changes in the Na indicators, cytokine concentration, NHE1 protein content, and NHE1 gene expression were determined. H 2 O 2 significantly promoted HUVEC injury, whereas ultrafiltered XMJ extract significantly improved the morphological changes in injured HUVECs, increased their activity, and decreased NHE1 gene and protein expression, as well as limited the decrease in membrane permeability and expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, interleukin (IL)-1, IL-6, and nuclear factor-kB. Ultrafiltered XMJ extract inhibited H 2 O 2 -induced HUVEC injury by inhibiting NHE1 activity.

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