No abstract

In connection with the toxicologic analysis of a number of parathion intoxications a method for determination of free and conjugated forms of p-nitrophenol (p-NP) as the main metabolite of parathion in blood and urine was established. Quantification of conjugates is based on their hydrolysis followed by detection of p-NP using a sensitive HPLC method. Hydrolysis of both p-NP-glucuronide and p-NP-sulfate is performed by specific enzymes and also by mineral acid, the latter is also found to be highly selective under definite conditions. The two hydrolysis methods applied showed a good correlation. The levels of free and conjugated p-NP in series of blood and urine samples were established after survival from two parathion intoxications. The individual levels of p-NP-sulfate and p-NP-glucuronide in both cases are discussed in respect of results made by other authors in this field.

p-Nitrophenol, the main metabolite of parathion metabolism can be quantified by high performance liquid chromatography (HPLC) following very simple preparation of small quantities of blood the urine. Determination is carried out by UV detection at 315 nm following separation on a RP 18 column. Detection limits are 5-10 ng/ml serum and 50-100 ng/ml urine. levels of p-nitrophenol in blood and urine after survival from parathion intoxication are demonstrated.

No abstract