No abstract

A method to eluat donororganspecific antibodies enabling direct and specific access to the pattern of humoral rejection after lung allotransplantation is demonstrated. The antibodies were evaluated quantitatively and qualitatively by elution of lung-grafts immunoglobulins and by a consequent investigation of the eluates using the passive haemagglutination- and indirect immunflourescence- test against donors' lung-antigen. Humoral antidonorlung-antibodies could be proved in all rejected grafts belonging to sensitized as well as to unsensitized recipients. There can be seen a highly significant correlation when comparing the results of the passive haemagglutination and indirect immunfluorescence test (r = 0,93, p less than or equal 0,01). On the other hand negative results of the investigations of eluates by lungantigens of other dogs show the specifity of humoral graft rejection. The suprising fact that there does not exist an essential difference between the results of sensitized and nonsensitized animals reveals a humoral immunresponse in hyperacute as well as in acute rejection.

Mit Hilfe eines biologischen Nachweissystems für das Enzym Kol-lagenase wurde die Aktivität dieser Protease an 12 invasiven Adeno-karzinomen des Kolons untersucht. Bei beträchtlichen quantitativen Unterschieden konnte in alien Fallen koUagenolytische Aktivität in den Dimensionen von 10^-3 Einheiten/mm 0 Gewebe festgestellt werden. EDTA hemmte die Reaktion um durchschnittlich 19%, normales menschliches Serum um 23 %. Dagegen ergaben 1,10–0-Phenanthrolin, D-Penicillamin sowie das Zytostatikum 5-Fluorourazil eine nahezu völlige Inhibition. Es kann geschlossen werden, daß die Aktivität des Kolonkarzinoms nicht aus Granulozyten, Serum oder normaler Mukosa, sondern aus dem Tumor selbst stammt. Im Gegensatz zur geringen Inhibierbarkeit durch normale Seruminhibitoren wird das Enzym durch 5-Fluorourazil nahezu völlig gehemmt.

No abstract

Using elution techniques, the humoral lung allograft rejection in immunosuppressively treated versus untreated recipients is analyzed at the donor organ specific level. The antibodies were evaluated quantitatively and qualitatively by elution of lung graft bound immunoglobulins and by testing the eluates against donor lung antigen using passive hemagglutination and indirect immunofluorescence. By correlating the results in those assays, a considerable amount of humoral anti-donor lung antibodies could by proved only in dogs not treated immunosuppressively, and there was no accordance with the results of direct immunofluorescence by quantification. The difference in the organ-bound antibodies between immunosuppressively treated and untreated lung grafts seems to be remarkable because of a similar mononuclear infiltration. Thus, enabling a specific improvement of some previous speculations about the lung-specific humoral alloimmune reaction, this type of rejection seems to be similar to rather stereotypical allograft rejection, but may be modified by a standard immunosuppression with methylprednisolone and azathioprine.