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The study was to assess the prevalence of extended spectrum beta-lactamases (ESBL) positive isolates from diarrheal stool samples in Namakkal District, Tamil Nadu (India). Totally 68 isolates were recovered from 125 samples and the isolates were identified as Escherichia coli by biochemical test and selective media. All isolates were subjected to determination of biofilm formation with tube method, among them 36 (53%) isolates were biofilm producers. The ESBL production was detected using a double disc synergy test and the presence of ESBL genes was evaluated by the Multiplex PCR method. In this study, 32.3% of isolates has been at least one type of ESBL genes, among them CTXm was most predominate gene. This result was acquainted with biofilm producers, and it was found that biofilm producing isolates mostly harbored the ESBL genes in comparison with non-biofilm producers.

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