Objective: A new simple, precise, rapid and selective high -performance thin-layer chromatographic (HPTLC) method has been developed for th e determination of lupeol in methanolic leaves extract of Andrographis echioides leavesResults: The retention factor of lupeol was found to be 0.55. Linearity was obtained in the range of 5000 ng -10000 ng for lupeol. The developed and validated HPTLC method was employed for lupeol in methanolic leaves extract of Andrographis echioides for standardization of the content of the marker. The linear regression data for the calibration plots showed a good linear relationship with r=0.99917 for lupeol. Satisfactory recoveries of 99.80 % were obtained for lupeol.. The concentration of standard lupeol was 1 mg/ml. Conclusion:The results obtained in validation assays indicate the accuracy and reliability of the developed HPTLC method for the quantification of lupeol in methanolic leaves extract of Andrographis echioides.

The present study was aimed to anti-inflammatory and anti-bacterial activities of titanium nanoparticles synthesized from Alpinia calcarata rhizomes of methanolic extract. Titanium nanoparticles exhibit brown colour in aqueous solution due to excitation of surface plasmon vibrations in Titanium nanoparticles. The appearances of creamy colour in the reaction vessels suggest the formation of Titanium nanoparticles. UV-Visible and FTIR spectroscopy are further confirmed the structural characterization and functional group identification of titanium nanoparticles. The SEM analysis showed the particle size 60-130 nm as well the spherical structure of the nanoparticles. Titanium nanoparticles is useful for the development of newer and more potent antibacterial agents. The synthesized nanoparticle further confirmed its anti-inflammatory and anti-microbial activities. All the above data represented in the study contribute to a novel and unexplored area of nanomaterials as medicine.

Objective: The present study is designed to develop a new simple, precise, rapid and selective high-performance thin-layer chromatographic (HPTLC) method for the determination of stigmasterol in methanolic rhizomes extract of Alpinia calcarata. Methods:As per International Conference on Harmonization (ICH) guidelines we have applied different concentrations of stigmasterol as standard on HPTLC plates for the quantification of stigmasterol from the Alpinia calcarata rhizomes. The concentration of standard stigmasterol is 1 mg/ml. Results:The retention factor of stigmasterol was 0.58. Linearity was obtained in the range of 50 ng-250 ng for stigmasterol. The developed and validated HPTLC method was employed for stigmasterol in methanolic rhizomes extract of Alpinia calcarata for standardization of the content of the marker. The linear regression data for the calibration plots showed a good linear relationship with r=0.99977 for stigmasterol, respectively Satisfactory recoveries of 99.77 % were obtained for stigmasterol. Conclusion:The results obtained in validation assays indicate the accuracy and reliability of the developed HPTLC method for the quantification of stigmasterol in methanolic rhizomes extract of Alpinia calcarata