In this study, various plants like Datura metel, Aegle marmelos, Annona reticulata and Saraca indica were collected and subjected for extraction using various solvents, namely Water, Chloroform and Ethanol. The extracts were done with TLC bioautography for Antioxidant activity and Antibacterial activity, Minimum Inhibitory Concentration, Antibacterial activity, Antioxidant activity (DPPH and FRAP) and Phytochemical analysis. Plants used in this study showed antibacterial and antioxidant activity. These extracts were further utilized for Silver nanoparticle production and were characterized. Silver nanoparticles were utilized for In vitro antibacterial activity, where they did not show any antibacterial activity.

The aqueous extracts of Euphorbia hirta was used to synthesise silver nanoparticles using bioreduction method. The nanoparticles were characterised by UV Vis spectroscopic analysis, SEM, EDX, AFM, XRD analysis. The silver nanoparticles were also tested for antibacterial activity in Pseudomonas aeruginosa and Bacillus subtilis. The minimum inhibitory concentration for the synthesised nano particles were also tested against the two bacterial species for the least concentration of 0.5µg/mL. The swarming motility assay and protein leakage assay was also tested for the nanoparticle. The silver nanoparticles were found to be much effective.

Silver nano particles synthesised from the leaves of Wrightia tinctoria were characterised by following instrumental analysis -UV Vis spectroscopy, Scanning Electron Microscopy, Energy dispersive X-ray spectroscopy, Atomic force microscopy and X-ray diffraction. The interaction of the silver nanoparticle to the microorganism was studied in Pseudomonas aeruginosa and Bacillus subtilis through agar diffusion method, minimum inhibitory concentration, swarming motility assay and protein leakage assay. The nanoparticles were found to be more effective against the bacteria used in this study.