Purpose and background: the aim of this study was investigating intraperitoneal injection of different doses of Imipramine on the number of follicles in the adult female rat. Infertility that occurs as a result of taking drugs is an important clinical issue. Rate of infertility that has been reported as a result of taking antidepressant drugs is 60%, in spite of its popularity a slight amount of studies has been formed to clarify the mechanism of action on these drugs, this study has written about Imipramine. forty female wistar 180 ± 20 g, selected and distributed them into groups experimental 1, 2, and 3. And they injected 6.25, 12.5, and 25 mg/kg bodyweight Imipramine respectively for 4 weeks, control group receives nothing, and sham group receives distilled water, at the end the samples of ovary tissue were taken to the laboratory for slice preparation, and slides of different parts of ovary made us available to count total number of follicles in each ovary. Results showed that this drug has no effect on bodyweight, ovary and primitive, secondary, graffian and atretic follicles. Different doses of imipramine, number of primary follicles, also 25 mg/kg dose of this drug has caused Corpusluteum to decrease.