The goal of the work was to obtain antibiotic-resistant forms of endophytic Glycine max L. (Merill) bacteria and to study their introduction potential affected by different seed treatment methods. Rifampicin-resistant variants of endophytic soybean bacteria Rhizobium radiobacter 27c and Pseudomonas fluorescens 11E preserving valuable properties were derived. Soybean seed treatment with Bradyrhizobium japonicum BIM V-501D and endophytic nitrogen-fixing Rh. radiobacter 27c, phosphate-mobilizing Ps. fluorescens 11E bacteria under model conditions promoted accumulation of nitrogen-fixing bacteria in the root, stem and leaves. The number of nodules rose by 70% compared with the mono-inoculated control; plant height increased by 19%.

High efficiency of introducing endophytic microbiota was demonstrated upon application of nitrogen-fixing and phosphate-mobilizing bacteria for pre-sowing treatment of winter wheat seeds.

The goal of the work was to study the effect of introducing nitrogenfixing and phosphate-mobilizing bacteria into liquid biohumus on its growth-promoting properties. The group of ammonifying microorganisms dominates in the structure of microbocenosis of liquid biohumus, constituting 98.46 %. The introduction of nitrogenfixing bacterial strains B. aryabhattai Cp-1 and phosphate-mobilizing Ps. Fluorescens Pr-2 at 10 % concentration in 1 % liquid biohumus (initial pH 9.5) changes pH to 7.8. Winter wheat seed treatment with liquid biohumus enriched with Ps. Fluorescens Pr-2 increases length and dry weight of seedlings by 40.0 and 80.2%; with B. aryabhattai Ср-1 – by 16.3 and 2.0% compared to the control (1% liquid biohumus). Application of liquid biohumus enriched with pseudomonades and bacilli for the treatment of cress-lettuce seeds provides increase of length and crude weight of seedlings by 25.7 and 20.0; 5.0 and 10.0%, respectively.