Influenza A virus is the most unpredictable global threat, past pandemic witnessed the community swaps happened due to this virus. The recent emergence of H7N9 in China is the result of new and recent reassortmant of Influenza A virus. Emergence of a novel human influenza A virus (H1N1pdm) in 2009 is an unprecedent event of molecular virology. This novel emerged virus affected millions of persons and raised WHO pandemic alert to phase 6. Therefore continuous monitoring is required to have adequate genome information and to track mutations during course of viral evolution. In present study we investigated acute phase suspected In the course of its evolution, 2009-2013 the virus acquired many mutations and which could be responsible for severity of this virus and emergence of new virus subtypes. India also witnessed high morbidity and mortality reported from all parts of the country. This study clearly indicates that recently reported cosmopolitan clade VII has undergone positive selection in India as well globally revealed continuous circulation of clade VII since 2009. This study of recent H1N1pdm Indian virus isolate will facilitate future epidemiological surveillance in Indian subcontinent and abroad.