A reproducible method is described for the determination of the MICs of ketoconazole, miconazole, fluconazole, and itraconazole with sharp endpoints when employed with either yeasts or molds. A semisolid medium is used with controlled pH and standardized inoculum. The time of reading results is a critical factor in the conduct of this test. The medium is simple to prepare and has a relatively long refrigerator shelf life in a user-ready state, requiring only the addition of a freshly prepared inoculum after restoration to room temperature.

Candida albicans is involved in periodontal disease, which is influenced by sex hormones. Aim: To study the effects of the estrogen antagonist tamoxifen (TAM) on periodontal disease of oncological patients; clinical oral strains of C. albicans. Patients: With periodontitis and breast cancer and other with AIDS were used. Materials & methods: Periodontal disease was evaluated by the academy of periodontology procedures and the growth of clinical C. albicans isolates were evaluated by the Clinical and Laboratory Standards Institute techniques. Results: Women who consumed TAM for more than 2 years decreased periodontitis severity. In vitro, TAM inhibited the growth of both fluconazole-sensitive and resistant C. albicans. Conclusion: Administered TAM chronically improves periodontal health and has antifungal activity on oral strains isolated from patients with odontologic and medical pathologies.

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