Soybean (Glycine max L. Merr.) seeds are highly sensitive to the environmental conditions experienced in storage. The objectives of this study were to identify QTLs for soybean seed storability by evaluating seed viability and seed vigor and investigating candidate genes for these traits in detected QTL regions. These objectives will aid in developing soybean cultivars with a high capacity for storability. The seeds of 109 and 90 recombinant inbred lines (RILs) derived from a cross between Misuzudaizu and Moshidou Gong 503 in 2010 and 2019, respectively, were used to evaluate seed viability by a germination test and seed vigor by an accelerated aging test, after storage under two temperature conditions (25 °C and 35 °C) for six months. Seed viability and seed vigor of Moshidou Gong 503 were higher than those of Misuzudaizu in both temperature conditions. The average seed viability and seed vigor of RILs decreased when stored at the higher temperature. A total of five QTLs were found for the two traits, seed viability and seed vigor, located in chromosomes A2, C2, and D1b. The Misuzudaizu alleles decreased seed viability and seed vigor at all detected QTLs. Most QTLs in this study were found near loci controlling seed viability, maturity, germination, seed hardness, and seed surface micromorphology, indicating that seed storability is related to these traits. In addition, two new QTLs were found that are associated with seed storability.

ABSTRACT:A suitable extraction solvent of seed protein for the identification of Thai rice varieties was determined for use in ultrathin layer isoelectric focusing (UTLIEF). Breeder seeds of 16 varieties were used and 3 extraction buffers, namely, phosphate buffer, NaCl, and water (with gel pH 2-9) were compared. The most suitable extraction buffer was phosphate buffer, which gave the highest efficacy of identification (98%) compared to NaCl (90%) and water (89%). The phosphate buffer was then tested in varietal verification using examination of individual seeds and testing bulk samples. The results show that the method of examination of individual seeds could clearly differentiate the contaminant variety from the tested variety, while testing bulk samples (10 seeds) could identify the contaminant variety when the ratio of seed number of the contaminant variety to the tested variety was 4-6 and higher. It can be concluded that phosphate buffer is suitable to be used in UTLIEF which is a promising method for varietal verification of Thai rice.

Soybean (Glycine max L. Merr. ) seeds are highly sensitive to the environmental conditions experienced in storage. The objectives of this study were to identify QTLs for soybean seed storability by evaluating seed viability and seed vigor and investigating candidate genes for these traits in detected QTL regions. These objectives will aid in developing soybean cultivars with a high capacity for storability. The seeds of 109 and 90 recombinant inbred lines (RILs) derived from a cross between Misuzudaizu and Moshidou Gong 503 in 2010 and 2019, respectively, were used to evaluate seed viability by a germination test and seed vigor by an accelerated aging test, after storage under two temperature conditions (25 °C and 35 °C) for six months. Seed viability and seed vigor of Moshidou Gong 503 were higher than those of Misuzudaizu in both temperature conditions. The average seed viability and seed vigor of RILs decreased when stored at the higher temperature. A total of five QTLs were found for the two traits, seed viability and seed vigor, located in chromosomes A2, C2, and D1b. The Misuzudaizu alleles decreased seed viability and seed vigor at all detected QTLs. Most QTLs in this study were found near loci controlling seed viability, maturity, germination, seed hardness, and seed surface micromorphology, indicating that seed storability is related to these traits. In addition, two new QTLs were found that are associated with seed storability.

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