Tuberculosis is a zoonotic disease caused by bacteria; Mycobacterium species. It is distributed worldwide and have the economic and public health significance. The present study was carried out to detect and identify the presence of Mycobacterium pathogens using polymerase chain reaction in blood samples from goats. Blood samples from the total of 236 goats suspected of tuberculosis as well as apparently healthy were collected from in and around Nagpur region. The DNA in blood sample was assessed by PCR amplification of IS6110 and RD1, RD4 gene to detect Mycobacteria at genus and species level respectively. The overall positivity of M. bovis among goats was recorded as 10.59% (25/236). The study demands thorough testing of the flocks for continuous monitoring for tuberculosis for preventing its further spread.

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