No abstract

Introduction: Periodontal ligament plays an essential role in preventing relapse after orthodontic treatment. Hyperbaric Oxygen Therapy (HBOT) and propolis gel can increase the amount of fibroblast in the tension area during orthodontic treatment, thus affecting the periodontal ligament. This research was aimed to analyse the difference of the width of the periodontal ligament and amount of fibroblast in the tension area with the administration of propolis gel and HBOT in an attempt to prevent orthodontic relapse. Methods: Forty-two male guinea pigs were randomly divided into 7 groups of treatments― the untreated group (I – negative group), group with rubber separator (II – positive group), 3% propolis gel treatment group (III), 5% propolis gel treatment group (IV), the HBOT treatment group (V), combination of 3% propolis gel and HBOT treatment group, and combination of 5% propolis gel and HBOT treatment group. The upper left central incisor was extracted distally using a 14-days separator rubber in the positive group and the treatment group; then the separator rubber was removed for 2 days to conduct the relapse process. The data were analysed by LSD statistical test. Results: The result of the combination of HBOT and propolis gel treatment showed significant differences among all groups (p<0.05) in the width of the periodontal ligament (1.03), and the number of fibroblasts was 95.67 in the tension site. Conclusions: The combination of HBOT and propolis gel affect the width of periodontal ligament and the number of fibroblasts in the area of the orthodontic relapse.

ABSTRACT

<strong><em>Background</em></strong><em>: The mechanical force of orthodontic device causes occurrence of a tension and a pressure area in the periodontal ligament. In the tension area, an inflammatory response characterized by vasodilation of blood vessels will occur. The function of blood vessel is to flow blood and other nutrients to accelerate the periodontal ligament remodeling process, so that relapse can be prevented. <strong>Objective:</strong> To determine the effect of combination of propolis 3%, 5% for 14 days and HBOT 2,4 ATA 3x30 minutes with 5 minutes interval for 7 days in the tension area of the periodontal ligament as an effort to prevent relapse. <strong>Materials and Methods</strong>: A randomized post-test only control group design was used in this study. 42 male guinea pigs (cavia cobaya) are divided into 7 groups K- (without treatment), K + (using separator), P1 (using separator and propolis extract 3%), P2 (using separator and propolis extract 5%), P3 (using separator and hyperbaric oxygen therapy), P4 (using separator and combination of hyperbaric oxygen therapy and 3% propolis extract), and P5 (using separator and combination of hyperbaric oxygen therapy and 5% propolis extract) . <strong>Results</strong>: Data shows that mean of diameter of blood vessels increased from the smallest K- (41,8417±0,84511), K+(61,77±1,254482), P1(72,5983±0,68869), P2(73,2333±1,24481), P3(74,2583± 0,62030), P4(91,47±0,76585), to the largest in P5(91,5483±0,96178). One-way Anova and LSD test showed significant differences in blood vessels diameter (p &lt;0.05). <strong>Conclusion</strong>: The combination of propolis 3% and 5% and HBOT have significant effect in increasing blood vessels diameter in the tension area of the periodontal ligament</em>