No abstract

According to TWGDAM guideline 4.5 (1), prior to implementing a new DNA analysis procedure or an existing DNA analysis procedure developed by another laboratory, the forensic laboratory must first demonstrate reliability of the procedure inhouse. Seven phases were designed to validate the use of the AmpFᐉSTR Profiler Plus PCR Amplification Kit, as well as the PE Applied Biosystems 310 Genetic Analyzer. This report summarizes the results obtained for each of the seven phases of the validation study which included the following evaluations: polymer, reproducibility, sensitivity, stutter, precision, mixtures and nonprobative casework.

A b s t r a c t The h a b i t a t u t i l i z a t i o n and movements of l a r g e n o u t h b a s s (Micro--p t e r u s s a l m o i d e s ) , y e l l o w p e r c h ( P e r c a f l a v e s c e n s ) , b l u e g i l l (Lepomis macrochirus) and pumpkinseed (Lepomis gibbosus) were s t u d i e d v i a r a d i o t e l e m e t r y . H a b i t a t u t i l i z a t i o n was c a l c u l a t e d a s t h e p e r c e n t a g e s of a t r a c k i n g -day each f i s h o c c u r r e d i n each h a b i t a t , weighted by t h e proport i o n a t e a r e a of e a c h h a b i t a t . The f i v e h a b i t a t s were heavy macrophyte (H), p r i m a r i l y Myriophyllum s p . ; l i g h t macrophyte ( L ) , p r i m a r i l y Potamogeton s p . ; sand ( S ) ; open w a t e r (O), having a n approximate minimum depth of 6 m and no macrophytes; e c o t o n e ( E ) , which was d e f i n e d a s 1.5 m on each s i d e of a boundary between macrophyte b e d s and sand o r open water. Movement speeds were determined by c a l c u l a t i n g t h e d i s t a n c e between two t r a c k e d l o c a t i o n s a t f i v e minute i n t e r v a l s . We examined h a b i t a t u t i l i z a t i o n of largemouth b a s s (Micropterus salmoides), yellow perch ( P e r c a f l a v e s c e n s ) , b l u e g i l l (Lepomis m a c r o c h i r u s ) , and pumpkinseed (Lepomis gibbosus) s i n c e such i n f o r m a t i o n could show which h a b i t a t s were most i m p o r t a n t t o t h e s e f i s h . We d e f i n e d h a b i t a t u t i l i z a t i o n i n a manner s i m i l a r t o t h a t employed i n s t u d i e s of mammals and b i r d s ( P i e t z and T e s t e r , 1982; S i n g e r e t a l . , 1981; and Gilmer e t a l . , 1975). I t was t h e p e r c e n t a g e of a t r a c k i n g day t h a t each f i s h occurred i n each h a b i t a t , weighted by t h e p r o p o r t i o n a t e a r e a of each h a b i t a t . While many f a c t o r s c o u l d i n f l u e n c e h a b i t a t u~i l i z a t i o n of t h e s e f i s h , p a r t i c u l a r l y w a t e r t e m p e r a t u r e (Warden and L o r i o , 1975; Ross and Winter,