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Medicinal plants are a type of plant that partially or completely and the exudate of the plant is used as medicine, medicinal ingredients, or medicinal ingredients. One of the plants that has been used by the community is Baban dotan (Ageratum conyzoides Linn.) which is one of the superior medicinal plants that has multifunctional properties. This study aims to determine the content of compounds contained in the Babandotan plant (Ageratum conyzodes Linn.) and analysis of the alkaloid content using the Thin Layer Chromatography Method. In the phytochemical screening examination of baban dotan (Ageratum conyzoides Linn.) plants. Examination of the analysis of alkaloids using the Thin Layer Chromatography method resulted in the results of the active substances in the Baban dotan plant in the form of alkaloid content separating or being attracted from the focal point and obtained 2 spots that appeared with a brownish yellow color. The results showed that in the phytochemical screening and alkaloid analysis of the Babandotan plant (Ageratum conyzoides Linn.) using the Thin Layer Chromatography method, there were characteristics that could be used to identify the plant.

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Senyawa golongan fenolik adalah senyawa aktif dari tanaman sirih yang berpotensi digunakan sebagai bahan obat alami. Penelitian ini bertujuan untuk menguji aktivitas sitotoksik senyawa golongan fenolik dari daun sirih (Piper betle L.). Aktivitas sitotoksik diuji dengan mengukur nilai LC50 menggunakan metode Brine Shrimp Lethality Test (BSLT). Ekstraksi dilakukan dengan cara maserasi dengan pelarut metanol 96%. Data yang diperoleh dianalisis dengan Probit Analysis. Hasil penelitian menunjukan bahwa senyawa golongan fenolik dari ekstrak metanol daun sirih memiliki aktifitas sitotoksik yang dengan nilai LC50 = 3,92 ppm dengan standart error 0,42 ppm.Kata kunci: Senyawa fenolik, sirih, Piper betle, sitotoksik

The objectives of the study are to investigated the growth-inhibiting and apoptosis mediating effect of Plectranthus amboinicus (Lour.) Spreng n-hexane extract (PANE) on T47D cell lines. The assays were performed in the study were cytotoxicity assay and apoptosis induction of T47D cells. The cytotoxicity effects were determined by using MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide] assay. The effect of apoptosis were observed by ethidium bromide-acrydine orange method. The growth of T47D was inhibited by treatment with PANE on concentrations 20; 40; dan 80 µg/mL. The cell death (apoptosis) induced by PANE was characterized by orange fluorescent on the IC50 concentration. These results concluded that the n-hexane extract of Plectranthus amboinicus, (Lour.) Spreng. inhibited the growth of T47D breast cancer cells in dose and time dependent manners. PANE also could induced apoptosis on T47D breast cancer cells, but the combination of PANE-doxorubicine did not show the synergistic effect. The results suggesting that PANE may be a potent agent for the chemoprevention of breast cancer.