The tasks of analyzing the processes underneath the integration of national legislation of Ukraine conforming with that of the European Union are critical for strengthening the State in quest of gaining membership in the European Union with the achievement of the strategic goals. This analytical article aims: 1) to unveil the genesis of the legal reform in the field of animal protection from ill-treatment and the use of animals for scientific purposes in Ukraine, 2) to analyze and summarize the features of regulations pertaining to the protection of animals from abuse within the EU, and 3) to outline further directions in reforming the domestic legislation of Ukraine concerning animal protection against ill-treatment and use of animals for scientific purposes in the context of European integration. Ukraine is gradually intensifying the process of reforming domestic legislation concerning cruelty to animals and use of animals for scientific purposes. The Verkhovna Rada (the Supreme Council) of Ukraine adopted Draft Law № 2351 of 30.10.2019, which still requires reformation to solve the highlighted problems. Some solutions are recommended for the Government of Ukraine.