Objectives: Carcinoma of the breast has commanded the interest of all the disciplines of medicine by magnitude and serious consequences of the lesion. In India the incidence of the disease is low in comparison to western countries. Cancer of the breast is the commonest canceraffecting woman worldwide. The objective of this study is to determine the relative frequency of genetic abnormality in cases of Breast carcinoma. Methods: The present study includes observations of 25 cases diagnosed as breast carcinoma from Gujarat cancer and research institute and Civil Hospital, Ahmedabad, during the year of 2014-15. About 30 metaphase plates were observed in each case and finally, a photograph was obtained from a good quality metaphase slide. Correlation of chromosomal finding was done with similar studies done in past. Results: The findings in present study suggest no structural and/or numerical chromosomal abnormality was detected in peripheral blood lymphocytes of any patients. Conclusion: The Karyotypic study helps to find out some numerical as well as structural chromosomal abnormalities and also useful in implementing a cost effective management of detecting early breast cancer in family members and to provide them with necessary preventive measures and treatment.