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An experiment of six weeks period was conducted to evaluate the effect of supplementing enzymes in soybean replaced with DDGS based diet on the performance and carcass characteristics of broiler chicks. The experimental work was conducted on 280 day old commercial broiler chickens randomly divided into seven dietary treatments having two replicates with twenty birds in each replicate, in each treatment group. Basal diet according to BIS (2007) was formulated by taking maize and soybean meal as main ingredients and was offered to control group (T 1 ), while treatment groups T 2 , T 3 and T 4 were supplemented with 15, 30 and 45% DDGS respectively, and T 5 , T 6 and T 7 were supplemented with protease enzyme @ 0.25% along with replacement of DDGS @ of 15, 30 and 45% respectively. The record of feed intake was done weekly on per replicate basis. The overall feed intake differed significantly (P<0.05). T 4 and T 7 groups having 45% DDGS replacement level with or without enzyme supplementation had higher (P<0.05) feed intake as compared to control diet. It was also observed that at 30% DDGS replacement level, body weight gain was significantly higher (P<0.05) due to addition of protease enzyme as compared to other dietary treatments. No significant effect observed on carcass traits of birds.