The purpose of this article was to review the fluoride release and uptake ability of some restorative materials which are used for minimally invasive procedures. Methods: The literature search on published researches and review articles were carried out by using PubMed, Trip and Cochrane library databases. The search terms used were fluoride, restorative materials, atraumatic restorative treatment or ART, glass ionomer or GIC, resin modified glass ionomer cement or RMGIC. The articles included were between 2000 and 2015. Conclusion: Fluoride release varies with m factors including the type of restorative materials used and also the media in which it is stored. Fluoride uptake is dependent on the type of the cement and the availability of fluoride including fluoride releasing capacity in the material.
Methanolic, ethanolic and ethyl acetate extracts of five aonla varieties were analyzed for phytochemical characterization using RP-HPLC. Five compounds viz. ascorbic acid, gallic acid, ellagic acid, ethyl gallate and quercetin were identified in aonla extracts by RP-HPLC at 270 nm. Significant variations were observed in amount of identified phytochemical among the varieties. The highest level of phytochemical was observed in methanolic extract of variety Desi followed by varieties Kanchan, NA-7, Banarasi and Chakaiya. Among the solvent, methanol extracted the maximum phytochemicals while yield was least in ethyl acetate extract of aonla varieties. Gallic acid and ellagic acid were the most abundant phenolic compounds in extracts of aonla varieties. Ellagic acid (349.51 mg/100 g) and gallic acid (233.49 mg/100 g) were found maximum in methanolic extract of Desi variety. Results of the present study suggested that aonla is a good source of phenolic and flavonoid compounds.
Phenolic content, antioxidant activities and antimicrobial activities of methanolic, ethanolic and ethyl acetate extracts of five different varieties of aonla () fruits as well their powders were evaluated. Total polyphenolic content in fresh aonla fruit extracts varied from 70.6 to 159.4 mg GAE/g and their EC 50 (effective concentration) values for antioxidant activity ranged from 46.72 to 359.7 µg/ml. Significant varietal difference were observed in antioxidant activity of the extracts of fresh aonla fruit and powder. Among the variety analyzed, Desi variety exhibited significantly higher TPC (total polyphenol content) and antioxidant activity in fresh as well as dried form in all the extracts. Methanolic extracts of various varieties had maximum TPC and antioxidant activity. Variety NA-7 showed high TPC and antioxidant activity. Almost, similar trend was observed among the extracts of aonla powders for TPC and AOA (antioxidant activity). A high positive correlation coefficient existed between TPC and AOA of different aonla extracts. All the extracts analyzed, exhibited a strong antimicrobial potential against and. This study suggests aonla as potential natural source of antioxidants and antimicrobial agents.
A Gram-stain-negative, rod-shaped bacterium, forming yellow colonies and designated CDR SL 15T, was isolated from the surface of Padina sp., a brown macroalga, which grows in the Western coastal regions of the state of Goa, India. The 16S rRNA gene sequence phylogeny placed the strain in the genus Luteimonas and it showed closest sequence similarity to Luteimonas terricola BZ92rT (97.6 %) and <97.0 % to other species of the genus Luteimonas. Chemotaxonomic features, such as having iso-C15 : 0 and summed feature 9 (C16 : 0 10-methyl/iso-C17 : 1ω9c) as the major fatty acids and Q-8 as the only ubiquinone further supported its placement within this genus. There were some critical differences in phenotypic properties between Luteimonas padinae sp. nov. CDR SL 15T and L. terricola DSM 22344T i.e. temperature range for growth and salinity range and optimum for growth (L. terricola is a psychrotolerant bacterium with a lower optimum temperature for growth), acid production and assimilation of substrates, enzyme activities and resistance to certain antibiotics. The DNA-DNA relatedness value of the novel strain with its closest phylogenetic relative was only 40 %, below the 70 % threshold value recommended for species delineation. All these characteristics are consistent with strain CDR SL 15T representing a novel species of the genus Luteimonas, for which the name Luteimonas padinae sp. nov. is proposed. The type strain is CDR SL 15T (=DSM 101536T=KCTC 52403T).
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