The paucity of report from Odisha regarding prevalence of ABO and Rh blood group promoted us to undertake the present retrospective study. Total number of subjects studied are 45,564. Prevalence rate of A, B and O are 9679 (21.24%), 13881 (30.46%) and 16662 (36.56%) respectively. The subgroup analysis showed of A1 and A2 has also been carried out in the present study. One case of A2 has been described which had antibody to A1 where there was difficulty in arranging the blood transfusion insisting there by the importance of subgroup analysis in the blood bank.

In vitro studies indicate that the uptake of ascorbic acid by skin, bone and teeth of rat declines with age. This may be correlated with the synthesis of collagen, the major constituent protein of the connective tissue. There is a decrease with age, in the synthesis of ascorbic acid due to alteration in enzyme systems. The concentration of total free ascorbic acid in various tissues, declines with age but at different rate. Fall of bound ascorbic acid contentduring aging, is probably due to crosslinking of proteins. The possible implications of theabove studies have been discussed.