Background: Gliomas are aggressive and resilient tumors. Progression to advanced stages of malignancy, characterized by cell anaplasia, necrosis, and reduced response to conventional surgery or therapeutic adjuvant, are critical challenges in glioma therapy. Relapse of the disease poses a considerable challenge for management. Hence, new compounds are required to improve therapeutic response. As hydrolyzed rutin (HR), a compound modified via rutin deglycosylation, as well as some flavonoids demonstrated antiproliferative effect for glioblastoma, these are considered potential epigenetic drugs. Objective: The purpose of this study was to determine the antitumor activity and evaluate the potential for modifying tumor aggressivity of rutin hydrolysates for treating both primary and relapsed glioblastoma. Methods: The glioblastoma cell line, U251, was used for analyzing cell cycle inhibition and apoptosis and for establishing the GBM mouse model. Mice with GBM were treated with HR to verify antitumor activity. Histological analysis was used to evaluate HR interference in aggressive behavior and glioma grade. Immunohistochemistry, comet assay, and thiobarbituric acid reactive substance (TBARS) values were used to evaluate the mechanism of HR action. Results: HR is an antiproliferative and antitumoral compound that inhibits the cell cycle via a p53- independent pathway. HR reduces tumor growth and aggression, mainly by decreasing mitosis and necrosis rates without genotoxicity, which is suggestive of epigenetic modulation. Conclusion: HR possesses antitumor activity and decreases anaplasia in glioblastoma, inhibiting progression to malignant stages of the disease. HR can improve the effectiveness of response to conventional therapy, which has a crucial role in recurrent glioma.

RESUMO Introdução:A dor lombar constitui causa frequente de morbidade e incapacidade, sendo a senescência patológica das facetas articulares da coluna vertebral uma das principais causas de lombalgia. Objetivo: Relatar os resultados obtidos no tratamento de pacientes idosos com lombalgia crônica através de analgesia peridural associada a rizotomia química percutânea de faceta. Método: O critério de inclusão neste estudo foi pacientes com presença de lombalgia crônica, sendo aplicada escala numérica de dor (END) para análise da dor pré-e pós-operatória no seguimento ambulatorial. Os pacientes foram submetidos a analgesia peridural seguida de rizotomia química percutânea de facetas articulares bilateralmente sob radioscopia intra-operatória. Resultados: Este estudo foi constituído de 19 pacientes. A pontuação média na END no pré e pós-operatório foi 8,2 e 1,9 pontos, respectivamente, com tempo médio de seguimento de 50 meses. Não houve óbitos ou complicações durante e após o ato cirúrgico, e os pacientes receberam alta hospitalar no mesmo dia da internação. Conclusão: São pontos favoráveis desta terapêutica: o menor tempo de internação hospitalar, segurança e baixo custo para execução. Esta associação de técnicas neurocirúrgicas apresenta-se como opção terapêutica promissora. Palavras-chave: Lombalgia; Manejo da Dor; Procedimentos Neurocirúrgicos; Idoso.ABSTRACT Introduction: Low back pain is a frequent cause of morbidity and incapacity and the spine facet joint dysfunction is one of the main causes. Objective: This paper aims to report the results obtained in the treatment of the elderly who suffer from chronic low back pain with epidural anesthesia associated to percutaneous chemical rhizotomy of facet joint. Methods: Inclusion criteria was patients who suffer from low back pain. The numerical scale of pain (NSP) was applied to analysis in the preoperative and postoperative pain in outpatients. The patients were submitted to epidural anesthesia associated to percutaneous chemical rhizotomy of facet joint bilaterally with intraoperative radioscopy aid. Results: This paper consisted of 19 patients. Mean scores were 8.2 and 1.8 points in the preoperative and postoperative period, respectively. The average follow-up was 50 months. There was no death or complication during and after the procedure, and patients were discharged on the day of admission. Conclusion: The shorter hospitalization stay, safety and low cost are favorable points of this therapy. This neurosurgical techniques association is presented as a promising therapeutic option for low back pain. IntroduçãoA dor lombar ou lombalgia é definida como uma condição clínica de dor regional moderada ou intensa, anatomicamente distribuída entre o último arco costal e a prega glútea. Frequentemente é acompanhada de limitações de movimento com etiologia variada podendo ser desencadeada por fatores de risco biológicos, mecânicos e cognitivos 21,50,57 . A lombalgia constitui causa frequente de morbidade e 274 Original Pacheco CC, Camporeze B, Estevão IA, Mariano PM, J...

Purpose: The purpose of this retrospective study was to evaluate the reliability of the free lateral arm flap in patients who had undergone defect coverage of the forearm and hand. Methods: From 1998 to 2006, the lateral arm flap was used to cover defect of the forearm and hand in 20 patients. The average age was 48 years, 14 patients were male and 6 were female. The etiology of the defects were in the majority of cases chronic or acute infections, often resulting from severe trauma. The maximum size of the defects ranged from 7 Â 4 cm to 15 Â 8 cm. Results: There were 15 patients with free flap reconstruction at the hand and five at the forearm level. All but one flap survived. In two cases a revision of the anastomosis was necessary due to venous congestion caused by a hematoma underneath the flap. In 18 patients primary closure of the donor site was possible. Eighty-five percent of the patients were satisfied with the result of the operation. Conclusion: Our results show that the lateral arm flap is an excellent option for reconstruction of defects at the forearm and hand. It has a reliable vascular anatomy which allows rapid flap dissection, a low donorsite morbidity and an excellent tissue quality for providing tendon gliding and good function. We strongly recommend this type of free flap for coverage of small and medium size defects of the forearm and hand.

selective condition, the effects of the mutation of interest can be analysed by measuring the proportion of each barcode in genomic DNA by real-time quantitative PCR or deep sequencing. Results and discussions We used this CRISPR-barcoding strategy to model different mechanisms of non-small-cell lung cancer resistance to EGFR inhibitors, and we established a multiplex system to evaluate the efficacy of combined drug therapies aimed at preventing or delaying the emergence of resistant cells. Through a similar approach, we assessed the effects of repairing oncogenic driver mutations in addicted cancer cells directly at the genome level. Finally, we used a highly complex set of CRISPR-barcodes to trace intratumor heterogeneity, as a convenient alternative strategy to lentiviral barcode libraries. Conclusion CRISPR-barcoding is a fast and highly flexible means to investigate the effects of different kinds of genomic modifications in a broad range of functional assays.

A metástase de carcinoma renal para região de sela túrcica é um evento raro, compreendendo menos de 1% dos casos de metástases para região de sela túrcica. Objetivo. O presente estudo relata caso clínico de metástase de carcinoma renal para região parasselar assistido pelo serviço de neurocirurgia do Hospital Universitário São Francisco de Assis na Providência de Deus - SP. Métodos. Relato de caso com base na análise retrospectiva do prontuário médico e dos exames complementares. Apresentação Clínica. Os autores relatam caso clínico de paciente apresentando história de ptose palpebral esquerda após cinco anos de ser submetido a nefrectomia radical esquerda devido ao diagnóstico de carcinoma renal. A ressonância magnética das órbitas e do encéfalo evidenciou presença de processo expansivo centrado na sela túrcica, ocupando e distendendo completamente a mesma e apresentando realce após injeção do contraste, resultante de metástase do carcinoma renal. A tomografia computadorizada do tórax demonstrou também haver metástases pulmonares e para linfonodos pré-traqueais, e a equipe optou por não realizar a intervenção cirúrgica. Conclusão. Metástase para região parasselar é uma variante rara de carcinoma renal pouco descrita na literatura, mas que deve ser considerada como diagnóstico diferencial das patologias localizadas nessa região.