Context: Albizia procera (Roxb.) Benth. (Mimosaceae) has been traditionally used in Thai longevity preparations. Thus, searching for HIV-1 integrase (HIV-1 IN) agents from natural sources is of interest. Objective: The objective of this study is to examine the inhibitory activity against HIV-1 IN of compounds isolated from the stem bark of Albizia procera. Materials and methods: The EtOH extract and isolated compounds of Albizia procera bark were examined for anti-HIV-1 IN activity at various concentrations (10-100 mg/mL and 10-100 mM) using the multiplate integration assay and molecular docking. Results and discussions:The results showed that the ethanol extract had good anti-HIV-1 IN activity with an IC 50 value of 19.5 mg/mL, whereas ethyl acetate fraction exhibited the most potent with an IC 50 value of 19.1 mg/mL, followed by water fraction (IC 50 value ¼ 21.3 mg/mL), hexane and chloroform fractions (IC 50 value4100 mg/mL), respectively. From bioassay-guided isolation, the ethyl acetate fraction was further separated to give two compounds which are (+)-catechin (1) and protocatechuic acid (2), respectively. Of the tested samples, (+)-catechin (1) exhibited appreciable activity against HIV-1 IN with an IC 50 value of 46.3 mM, whereas protocatechuic acid (2) showed mild activity with 46.0% inhibition at concentration of 100 mM. (+)-Catechin (1) could interact with Thr66, Gly148, and Glu152 in the core domain of IN enzyme, whereas protocatechuic acid (2) could bind with Thr66, His67, Glu152, Asn155, and Lys159. This is the first report on anti-HIV-1 IN activity of Albizia procera bark. These results may suggest that Albizia procera bark has potential as anti-HIV-1 IN agent.