No abstract

The materials used were ethanol, soluble starch, commercial corn starch, tryptone, yeast extract, sodium carbonate, sodium chloride, and agar. β-cyclodextrin and HPLC grade acetonitrile were purchased from Sigma-Aldrich Ltda, São Paulo, Brazil. The antibiotics used were Kanamycin Sulfate and Hygromycin B., from Streptomyces hygroscopicus. All other reagents used were of analytical or chromatographic grade. Methods Cultivation and growth of the recombinant B. subtilis WB800 bacteriumFor the cultivation and growth of the bacteria, 2xYT medium (liquid and solid) was used, composed of 1.6% tryptone, 1% yeast