Zooplankton grow in the last stage of water puri®cation at the Werribee Sewage Treatment Lagoons (WSTL) and the resource is huge and unutilized. Daphnia carinata and Moina australiensis are the dominant species at the WSTL. The protein content of D. carinata and M. australiensis was 54.80% and 64.80%, respectively. Analysis of zooplankton showed that both essential and nonessential amino acids were present at a level that was higher than control diet. Silver perch fed on D. carinata exhibited better growth, better food conversion ratio (FCR), protein eciency ratio (PER) and apparent net protein utilization (ANPU), which were not signi®cantly dierent from the control diet (P > 0.05). Heavy metals concentrations were at very low levels in ®sh fed on wastewater-grown zooplankton.

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Although botulinum toxin injection was well tolerated, these results using Dysport at a dose of 240 mouse units question its efficacy as a treatment for achalasia.