The vertical jump is a complex movement where many factors are involved in the final result. Currently, how a specific exercise program for gluteal muscles can affect the vertical jump is unknown. So, the aim of this study was to examine the effect of a specific exercise program for the gluteal muscles on a vertical jump. Forty-nine amateur athletes completed an 8-week program. The experimental group received a specific gluteal muscle training program in addition to their regular training routine, whereas the control group received their regular training routine. Jump height, flight time, speed and power were assessed (baseline, postintervention, and 4-week follow-up). Repeated-measures analyses of variance were conducted with ∝ ≤ 0.05. We calculated Eta squared effect sizes with 95% confidence intervals. Measurements at 8 weeks revealed significant increases in the experimental group compared to the control group for the values: jump height (p < 0.05) (experimental group = 17.15%; control group = 3.09%), flight time (p < 0.001) (experimental group = 7.98%; control group = 3.52%), speed (p < 0.01) (experimental group = 1.96%; control group = 1.83%) and power (p < 0.05) (experimental group = 4.43%; control group = 0.32%). However, at follow-up, these changes were not maintained. These data suggest that this specific training protocol for the gluteal muscles is effective in order to improve vertical jump performance in amateur athletes who use the vertical jump in their routine training habits.