O papel da narrativa como meio de conhecimento é valorizado há muito tempo por diversas disciplinas como a história, a psicologia, a filosofia, a linguística, a antropologia ou a literatura. Durante as últimas décadas, também a educação passou a reconhecer, de forma crescente, a importância da narrativa como metodologia de investigação e de desenvolvimento pessoal e profissional de professores. Este artigo pretende apresentar e ilustrar algumas potencialidades da utilização de narrativas em diferentes contextos educativos. Para tal, recorre a diferentes narrativas elaboradas por professores e alunos participantes em diferentes projectos de formação e de investigação coordenados pelo seu autor.
Hormones play an important role in fruit ripening and in response to biotic stress. Nevertheless, analyses of hormonal profiling during plant development and defense are scarce. In this work, changes in hormonal metabolism in grapevine (Vitis vinifera) were compared between a susceptible (Trincadeira) and a tolerant (Syrah) variety during grape ripening and upon infection with Botrytis cinerea. Infection of grapes with the necrotrophic pathogen Botrytis cinerea leads to significant economic losses worldwide. Peppercorn-sized fruits were infected in the field and mock-treated and infected berries were collected at green, veraison and harvest stages for hormone analysis and targeted qPCR analysis of genes involved in hormonal metabolism and signaling. Results indicate a substantial reprogramming of hormonal metabolism during grape ripening and in response to fungal attack. Syrah and Trincadeira presented differences in the metabolism of abscisic acid (ABA), indole-3-acetic acid (IAA) and jasmonates during grape ripening that may be connected to fruit quality. On the other hand, high basal levels of salicylic acid (SA), jasmonates and IAA at an early stage of ripening, together with activated SA, jasmonates and IAA signaling, likely enable a fast defense response leading to grape resistance/ tolerance towards B. cinerea. The balance among the different phytohormones seems to depend on the ripening stage and on the intraspecific genetic background and may be fundamental in providing resistance or susceptibility. In addition, this study indicated the involvement of SA and IAA in defense against necrotrophic pathogens and gains insights into possible strategies for conventional breeding and/or gene editing aiming at improving grape quality and grape resistance against Botrytis cinerea.
Seine biologische Rolle ist noch unklar, dennoch zeichnen sich für den natürlich vorkommenden Vanadiumkomplex Amavadin (1) bereits industrielle Anwendungen ab: Amavadin (und ebenso andere VIV‐ und VV‐Komplexe mit N,O‐ und O,O‐Liganden) können die direkte Umwandlung von Methan in Essigsäure katalysieren – die Reaktion verläuft in Abwesenheit von CO unter sehr milden Bedingungen und mit hohen Ausbeuten (siehe Schema).
25Botryosphaeria dieback is a grapevine trunk disease with a worldwide distribution and associated 26with Diplodia seriata and Neofusicoccum parvum among several other Botryosphaeriaceae species. 27The aforementioned xylem-inhabiting fungi cause wood lesions, leaf and berry symptoms and 28 eventually lead to the death of the plant. The aim of this work was to develop a simple model 29 system to reproduce the foliar symptoms caused by D. seriata and N. parvum to better characterize 30 fungal pathogenicity and determine the mechanisms involved in symptom development. This paper has been peer reviewed and accepted for publication but has not yet been copyedited or proofread. The final published version may differ. 3Several Botryosphaeriaceae species are associated worldwide with the grapevine trunk diseases 45 (GTDs) known as Botryosphaeria dieback (Moller and Kasimatis 1978; Larignon and Dubos 1997; 46 Graniti et al. 2000; Fischer 2006;Larignon et al. 2009;Úrbez-Torres 2011; Spagnolo et al. 2014a; 47 Larignon et al. 2015). The most common species isolated from grapevine-growing regions around 48 the world include Diplodia seriata De Not. (Cristinzio 1978; Rovesti and Montermini 1987; 49 Larignon et al. 2001; Castillo-Pando et al. 2001; Phillips et al. 2007; Savocchia et al. 2007; Úrbez-50 Torres et al. 2008) and Neofusicoccum parvum (Pennycook & Samuels) Crous, Slippers & A.J.L. 51Phillips (Crous et al. 2006). These fungi are xylem-inhabiting and attack the framework of 52 grapevines causing perennial cankers in the wood, resulting in leaf and berry symptoms and finally 53 leading to the death of the plant. Symptoms are characterized by yellowish-orange (white cultivars) 54 or wine-red (red cultivars) spots on leaf margins and blade, and in most cases, the emergence of a 55 brown stripe on the wood under the bark (Larignon et al. 2001; Spagnolo et al. 2014a). This 56 symptom is often associated with a grey sector of rotted wood. Shrivelling and drying of 57 inflorescences or fruit clusters are frequently observed. 58The incidence of Botryosphaeria dieback, together with two other trunk diseases, esca and 59Eutypa dieback, has increased over the years. In France it was estimated that 13% of productive 60 vines were affected by GTDs in 2012 (Grosman and Doublet 2012; Bruez et al. 2013). Although 61 GTDs, including Botryosphaeria dieback, appear to be increasingly common, accurate knowledge 62 of host-pathogen interactions poses certain problems, including (i) determining the seasonal 63 influence on field-collected data due to an uncontrolled environment and (ii) distinguishing 64 pathogen effects on grapevines from effects in response to other biotic agents in the field. This paper has been peer reviewed and accepted for publication but has not yet been copyedited or proofread. The final published version may differ. 4Eutypa dieback symptoms, the stunting of new shoots with small cup-shaped, chlorotic and tattered 70 leaves, were reproduced on greenhouse cuttings that were infected with Eutypa ...
Synthetic amavadine models, [V(HIDPA) 2 ] 22 and [V(HIDA) 2 ] 22 [where HIDPA and HIDA stand for the basic forms of 2,2A-(hydroxyimino)dipropionic and 2,2A-(hydroxyimino)diacetic acid, respectively], exhibit haloperoxidase-or peroxidase-type activities, and act as catalysts for the selective peroxidative monohalogenation, hydroxylation or oxo-functionalization of alkanes or aromatic compounds such as benzene and mesitylene at room temperature.
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