A laminated shell microcapsule is described resisting aggregation and withstanding ultrasound destruction, showing a good backscatter signal, as shown in the figure. Templated synthesis produces versatile monodisperse capsules <3 µm, with ultrasound‐pressure dependency allowing rupture above MI ∼ 1.5 (at 2 MHz), suitable for future development as both controlled‐delivery agent and contrast agent.

Background: Mutations in PAX3 cause Waardenburg syndrome (WS), but the mechanism remains unclear. Results: PAX3 binds mitotic chromosomes using its homeodomain (HD), a process that depends on arginine methylation and is lost in WS. Conclusion: PAX3 with mutations in HD causes WS by failing to load on mitotic chromosomes. Significance: This study pinpoints the molecular defects of a specific group of PAX3 WS mutants.