This study aims to synthesize hollow microspheres (HMS) from rape pollen via H3PO4 hydrothermal carbonization. The rape pollen hollow shell was used as the carrier and bovine serum albumin as a model protein. The properties of HMS were characterized by scanning electron microscope (SEM), solid-state nuclear magnetic resonance and elemental analysis. The SEM images clearly showed that the HMS had perfect spherical morphology and porous hollow surface. In the separated filtrate, a large number of sucroses were detected by high-performance liquid chromatography, suggesting that the hydrolysis of starch molecules occurred during the hydrothermal process. The formation of HMS was that the rape pollen inclusion was removed from rape pollen shell to preserve integral HMS by H3PO4 hydrothermal. The HMS possessed amphiphilic surfaces, which was suitable for protein adsorpion and pH-controlled release application.

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Background: The ketogenic diet (KD) has been implemented in many different counties. However, in China, study concerning the efficacy of the KD is still at an early-stage of evaluation. Furthermore, the KD is thought to be incompatible with Chinese children because of its lack of palatability, especially for the Asian population. In addition, its substantial antiepileptic effect remains to be confirmed.Methods: To evaluate the efficacy and safety of the KD treatment of refractory childhood epilepsy in China, we prospectively enrolled 147 children with refractory epilepsy for KD treatment in Guangdong 999 Brain Hospital and followed up the children for 6 months. Outcome was measured by seizure frequencies before and after the KD diet and adverse effects. We also evaluated influences of different variables (starting age, duration of epilepsy, and others) on the outcome. Results:We found after 1 month, 3 months, and 6 months of KD treatment, 28.0%, 55%, and 67.9% of the subjects remained on diet with a >50% seizure reduction and seizure-free rates of 6.5%, 13.2%, and 23.3%, respectively. Gender, starting age, duration, etiology, classification, and seizure type of epilepsy showed no significant influence on efficacy. Anorexia, diarrhea, and gravel were the main side-effects of the KD treatment. Conclusions:In conclusion, the KD is a safe and efficacious method for childhood refractory epilepsy treatment.

Transit-oriented development (TOD) is among the most feasible strategies for relieving urban issues caused by the unbalanced development of transportation and land use. This study proposes a multiobjective TOD land use design framework for the optimization of the land use layout in station catchments. Given the high density and diverse development in Chinese megacities, a planning model that considers nonlinear impacts on ridership, land use efficiency, quality of life, and the environment is constructed. The model applies fine-grained geo-big data to fill gaps in the empirical and statistical data and improve practicability. A genetic multiobjective optimization approach without reliance on objective weighting is used to generate alternative land use schemes. A metro station in Shanghai is applied as a case study. The results indicate that the proposed ridership objective outperforms the commonly used linear function, and the optimization method has superior extreme optima and convergence to baseline models. We also discuss the consistencies and conflicts in the objectives and provide a balanced land use scheme considering local policies. This work provides suggestions for sustainable urban design with coordinated land use and transportation.