In this study, the high-temperature test (i.e., temperature to 1000 °C) is conducted on 600 MPa seismic steel bars, and its residual mechanical properties and constitutive relations are investigated though three cooling rates, i.e., under air, furnace, and water-cooling conditions. Results show that three cooling methods have significant effects on the apparent characteristics of 600 MPa steel bars, when the heating temperature is greater than 600 °C. In addition, the ultimate and yield strength of steel bars have been significantly affected by different cooling methods, with increasing heating temperature. However, the elastic modulus is significantly not affected by temperature. Furthermore, the elongation rate after fracture and the total elongation rate at the maximum force do not change significantly, when the heating temperature is less than 650 °C. The elongation rate, after fracture, and the total elongation rate, at the maximum force, have different changes for three cooling methods. The degeneration of the stress–strain curves occurs when the heating temperature is high. The two-fold line, three-fold line, and Ramberg–Osgood models are developed based on the stress–strain curve characteristics of steel bars after cooling. The fire resistance of 600 MPa steel bars of reinforced concrete structure is analyzed, which provides a basis for post-disaster damage assessment, repair, and reinforcement of the building structure.