Histology Staining Procedure for tissues from ambulatory dogs after subcutaneous nerve stimulation.

Immunostaining of tissue sections from dogs with subcutaneous nerve stimulation.

This animal protocol was approved by the Institutional Animal Care and Use Committee of the Indiana University School of Medicine and the Methodist Research Institute, Indianapolis, IN, and conformed to the Guide for Care and Use of Laboratory Animals. The surgical procedure is performed under anesthesia and should incorporate all local requirements for standards of animal experimentation, including methods of anesthesia, surgical environment, and post-operative monitoring and care.