No abstract

In this study, several thin-layer chromatography (TLC) methods are described for the identification of quaternary and non-quaternary compounds (parent compounds, intermediates, by-products, and products) arisen within the synthesis of the acetylcholinesterase reactivator HI-6, at present the most promising antidote in the case of nerve agent poisonings. Using the TLC technique, particular E and Z isomers of this compound on the oxime group are separated. These TLC methods could be of high interest as quick purity control for those who are interested in development of new acetylcholinesterase reactivators and the synthesis of HI-6 in laboratories or in large-scale production.

Quaternary pyridinium salts with chains ranging from C8 to C20 belong in the large group of cationic surfactants. In this paper, the preparation of such cationic surface active agents based on the pyridinium moiety and differing in the length of the N-alkyl chain is described. Additionally, HPLC technique was established to distinguish each prepared pyridinium analogue. This study represents universal method for preparation and identification of quaternary pyridinium detergents.