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Xylose reductase from the xylose-fermenting yeast Pichia stipitis was purified to electrophoretic homogeneity via ion-exchange, gel and affinity chromatography. At physiological pH values the thermodynamic equilibrium constant was determined to be 0.575x101° (1-mol-1). Product inhibiton studies are reported which clearly show that the kinetic mechanism of the xylose reductase is ordered-bi-bi with isomerisation of a stable enzyme form.

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The potential of microbial degradation of PCB in contaminated actual site soil was investigated by incubation in percolation columns for 10 months. The addition of traces of mineral salts and nutrients resulted in a significant increase of the degradation of PCB congeners up to 5 Cl-atoms caused by the naturally occurring bacteria in the soil matrix. If only tap water was recycled the degree of PCB degradation was negligible.

No abstract