Background and Aim Biomarkers of pesticide exposure are generally lacking in communities where agricultural crops are grown. The purpose of this study was to focus on evaluating biomarkers of pesticide exposure in people living in an agricultural area of San Pa Tong District in Chiang Mai Province, northern Thailand. Materials and Methods One hundred and twenty-four participants (38 nonfarm workers, 38 rice growers, 31 longan growers, and 17 vegetable growers) from San Pa Tong District gave consent to participate in the study. Pesticide exposure was assessed by determining acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) levels in blood samples using Ellman's method and measuring 6-dialkylphosphate metabolites (DAPs), 3-phenoxybenzoic acid (3-PBA), and glyphosate in urine samples using chromatographic methods. Results AChE and BChE activities in the nonfarm worker group had higher level than those in the grower groups. DAPs were detected in almost all urine samples and 3-PBA was detected in 12-45% of each group, while glyphosate was found in 11 – 30% among the three groups of growers but not in nonfarm workers. Conclusion In this study, participants living in an agricultural area of San Pa Tong District were exposed to organophosphate, synthetic pyrethroid, and glyphosate through multiple pathways.