The problem of replica plating filamentous fungi such as Coprinus lagopus is overcome by inducing micro-colonies with sodium deoxycholate and using ' Velcro', a hooked material, to replace velveteen in the standard replica plating technique. ' Velcro' is advantageous in that it has a regular pattern of closely spaced hooks which transfer small inocula from the colonies on the master plate.* Present address: School of Biological Sciences, University of East Anglia, Norwich.