The aim of this study was to investigate the tyrosinase inhibitory and antioxidant activities of the extracts and constituents of Paeonia lactiflora Pall flowers 'Madame de Verneville'. The 70% ethanol extract was purified by combination of chromatographic methods to afford twenty-six known compounds. Their structures were elucidated based on 1D and 2D nuclear magnetic resonance (NMR) spectra and mass spectrometry. High performance liquid chromatography (HPLC) analysis showed that the dominant compounds were 1,2,3,4,6-penta-Ogalloyl--D-glucopyranoside ( 16) and 6-O-m-digalloyl-1,2,3,4-tetra-O-galloyl--D-glucopyranoside (19). The in vitro fungal tyrosinase inhibition potential and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of the extract and isolated compounds were evaluated. 70% EtOH extracthas a high tyrosinase inhibitory and good antioxidant activities (IC 50 = 0.35 and 2.3 µg•mL -1 , respectively). Compound 15 (1,2,3,6-tetra-O-galloyl--D-glucopyranoside) showed high tyrosinase inhibitory and antioxidant activities (IC 50 = 0.23 and 3.1 µg•mL -1 , respectively).