The aim of this study was to determine the relationship between the isokinetic characteristics of knee extensors and flexors with selected motor abilities: Speed, agility, and explosive power of lower extremities of professional football players in the preparation period of a yearly training cycle. Twenty-one players (age: 24.5 ± 3.9 years; body mass: 76.7 ± 4.7 kg and body height: 183.5 ± 5.5 cm) playing in the highest Polish soccer league participated in the study. The isokinetic concentric torque of the knee extensors and flexors was measured at 300°/s, 180°/s, and 60°/s velocities. Sprint performance was assessed in the 30 m sprint test (standing start). The forward, lateral, and backward movements were assessed using the T-Test of agility. Explosive power was quantified by performing the squat jump (SJ) and countermovement (CMJ), using the force platform. Due to sport-specific demands of soccer activities measured in this experiment, the relationships between peak torque (PT) and the 30 m sprint, T-Test of agility, and power of vertical jumps (SJ and CMJ) were low or medium at speeds of 60°/s and 300°/s. One of the main reasons for the lack of high dependence of the above-mentioned factors are that the measurements were performed during the initial training period where the level of individual abilities is at a low level. Additionally, this experiment may also indicate that the measurement of isokinetic knee flexion and extension peak is effective when performed at the correct angular velocity in relation to the evaluation of the intended motion structure.