Objectives: To estimate the knowledge of "Immunization of Health Care Workers" in field practice area of Jamdoli, Jaipur among paramedical staff regarding Hepatitis B, Polio, Meningitis, Influenza, Measles, Diphtheria, Rubella, Varicella and to assess the factor associated with the knowledge about immunization among the paramedical staff. Materials and Methods: It was a Cross sectional study conducted in conducted in field practice area of Jamdoli, Jaipur among paramedical staff from October 2018 to December 2018. The sample Size was calculated from Prevalence of adequate knowledge of Polio Vaccination is 91% which was 131 so total sample size is 135. Convenience sampling method was adopted and Pretested predesigned structured interview schedule was conducted as study tool. Results: The majority (40%) paramedical staff enrolled in this study were in the age group of 20-30 yrs. The male and female respondents were 14.1% and 85.9% respectively, 63% respondent belongs to general category. The majority (91.1%) of the respondent qualified up to GNM with serive duration (45.9%) more than 10 years. Regading Route of transmission, for Hep B (78.5%) answered parenteral, Polio (49.5%) answered faecooral, Diptheria (81.5%) droplet etc. The highest knowledge was about Hepatitis B 91.9% and the poorest was regarding rubella 33.3%. Satisfactory knowledge among the respondents were 55.6% depending upon mean score (26.7). The satisfactory knowledge significantly associated with the caste of the respondents (P= 0.02). Conclusion:Education and Communication strategies for improving the level of knowledge and for the immunization uptake regarding Hepatitis B, Polio, Meningitis, Influenza, Measles, Diphtheria, Rubella, Varicella among health care workers are strongly needed.