In present research work Sixteen different isolates of Xanthomonas axonopodis pv. citri were tested on different acid lime cultivars. The pathogenicity was confirmed by reaction of these isolates on different citrus cultivars by inoculation. It was found that variety Chankradhar was most susceptible to all the isolates of Xanthomonas axonopodis pv. citri and expressed symptoms in 3 -10 days after inoculation. Xac1 and Xac12 produced symptoms on cultivar Chankradhar 3 days after inoculation followed by Xac 2, Xac11 and Xac16. However, only two Xac isolates viz. Xac12 and Xac 13 were produced symptoms on cultivar Bagalipattu and expressed symptoms within 4 day of inoculation. The isolate Xac12 and Xac14 represented distinct nature of virulence as it initiated water soaked lesion within three to six days. Tahiti was found resistant against all isolates as none of the isolate was able to cause the disease.
In present research work eight different isolates of Xanthomonas axonopodis pv. citri were tested on different acid lime cultivars. The pathogenicity was confirmed by reaction of these isolates on different citrus cultivars by inoculation. The water soaked lesions along with fully developed symptoms were developed after 9 to 12 days of inoculation. The Xac1 was highly virulent and Xac2, Xac4, Xac5, Xac6 were poor virulent and Xac3, Xac7, Xac8 were moderately virulent.
Bacterial blight disease of pomegranate caused by Xanthomonas axonopodis pv. punicae is one of the most destructive disease of pomegranate (Punica granatum). Study was undertaken to conduct collection of samples, isolation, identification, pathogenicity, study of different characters of pathogen, morphological variability, cultural variability and biochemical variability of selected isolates and in vitro evaluation. The isolations were made from the symptomatic samples collected from different districts of Maharashtra (India). The studies takes for all eight isolates on gram staining was showed gram negative reaction. The studies on biochemical characteristics of Xanthomonas axonopodis pv. punicae showed their positive reactions for potassium hydroxide (KOH) solubility test, starch hydrolysis test, Gelatin liquification, H2S Production, catalase test, oxidase test, Tolerance to 1% NaCl whereas, negative response for indole production, urease test, Arginine test Methyl red test.
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