A near‐infrared chemodosimeter based on an aza‐BODIPY dye was designed and synthesized. The sensor contains isothiocyanate groups for cyanide ion sensing. The sensing function was illustrated via the fluorescence changes in near‐infrared frequencies as well as chromogenic changes which could be easily visualized with a detection limit of 19 ppb. The sensor provides high selectivity to CN− and discriminates other anions such as CH3COO−, HPO4−, HSO4−, ClO3−, CO32−, SO42−, NO3−, Cl−, F−, Br−, I−, and phenylalanine (Phe) in 50 % PBS buffer/acetonitrile at physiological pH. The potential of the sensor for CN− detection in both aqueous buffer solutions and living cells imaging was demonstrated.
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