, and create public health importance, in acute diarrhea diseases. Epidemic of cholera have been reported from various parts of our country. (1)(2) A total fecal specimen 562 were collected, isolation and identification of pathogen was done according to standard methodology. (3) Antibiotic susceptibility pattern of the isolates were studied, and picked up randomly positive 25 isolates and were sent to national institute of cholera and enteric diseases (NICED) Kolkata for confirmation and Phage typing. RESULTS: Of the 562 of patients 57 (10.142%) isolates were found to be positive for vibrio. Cholera o1 and non 01/non-0139, out of 25 isolates 19 (76%). The vibrio Cholera 01 ogawa serotype, biotype Eltor and phage type T 27, T 24, T 21, T 13, along with 6 isolates (24%) of Non-0139 was also noticeable as per the report of the (NICED) Kolkata. Majority of the clinical isolate were found to be resistant to more than one drug. (1)(2)

AIM:To identify the Seropositivity of dengue virus infection in clinically suspected patients. MATERIAL AND METHODS: A cross sectional study was conducted in north Karnataka from Jan 2012 to August 2013. Clinically suspected patient suffering from dengue virus infection were included in the study according to WHO criteria. Acute phase sera were collected after 5 days of the onset of fever as per WHO criteria. Serology was performed using ELISA for detection of IgG and IgM. A primary dengue infection was defined by a positive IgM ELISA and negative IgG. Secondary infection was defined as positive IgG with or without IgM. RESULTS: Among 580 patient who met the WHO criteria for dengue infection 246(42.41 %) were ELISA positive among whom 71(28.87%) had primary infection. Secondary infection was reported in 175(71.13). Acute phase sera of 344 (59.31%) were negative by ELISA. Thrombocytopenia was evident in 52(71.83%) with primary infection and 115(65.71%) patients with secondary dengue infection out of 167 patients who were positive for dengue IgM/IgG antibodies.

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