An experiment was aimed to assess the use of the legumes as a source of protein feedstuff and levels of protein-energy synchronization (PES) index in the diet of dairy cattles on volatile fatty acids (VFA) and methane (CH4) profile. The research was applied in In vitro techniques used a completely randomized design (CRD), with factorially pattern (2x3), the first factor was the two species of legumes (Sesbania and Leucaena) and the second factor was the three level of the PES index (0.4, 0.5, and 0.6), there were 6 treatment combinations and each was 4 replicates. The results showed that interaction between legumes with PES index was not significantly affected (P>0.05) on all variable. Legumes was not significantly affected (P>0.05) on all variables and PES index was significantly affected (P<0.05) on propionate, A:P ratio, and methane. The study concluded that the use of turi and lamtoro leaves combined with the protein-energy synchronization index (PES) at medium level (0.5) could increasing the production of VFA, especially propionate, suppressing A:P ratio and decreasing methane production.

Protein is one of the nutrient components in milk that is related to product quality. The components of milk protein are divided into casein alpha-s1, beta, alpha-s2, kappa, and whey fractions such as alpha lactalbumin and beta lactoglobulin. There are no existing data of milk protein fraction in dairy cow and goats in Banyumas Regency. This study aimed to determine the profile in form of protein fractions of cow and goat milk in Banyumas. Milk sample from fifty cows and thirty dairy goats was taken by random sampling in some areas. The milk protein profile was identified by the technique of sodium dodecyl sulphate gel electrophoresis (SDS-PAGE) and protein quantity prediction by software. The data obtained were analyzed statistically by Mann-Whitney between cows and goats. The results were significantly different (p<0.05) between cows and goats in molecular weight of protein alpha-S1 casein (29.66 vs 33.37 kDa), alpha-S2 (27.76 vs 29.49 kDa), beta (24 , 48 vs 25.59 kDa) and beta lactoglobulin (15.75 vs 15.97 kDa). The quantity of casein alpha-S1 (7.88 vs 4.16 g/l), alpha-S2 (1.31 vs. 4.02 g/l), beta (8.74 vs 14.24 g/l), kappa (2.41 vs. 4.28 g/l) and alpha lactalbumin (0.91 vs 0.7 g / l) was significantly different (p <0.05) between cow's and goat's milk, respectively. In conclusion, milk protein profile of cows and goats in Banyumas Regency is different.

ABSTRAKAgar keberlanjutan kehidupan manusia dapat berlangsung dengan baik, diperlukan berbagai dukungan, salah satunya adalah pemenuhan kebutuhan protein hewani. Salah satu pemasok sumber protein hewani adalah ternak ruminansia dengan produk berupa susu dan daging. Kebutuhan pakan utama ternak ruminansia adalah hijauan yang dapat dipasok dari tanaman sliridia (Glerisia maculata), yang salah satunya tumbuh di pantai Petanakan, Kabupaten Kebumen. Untuk mengetahui bagaimana kondisi tanaman Sliridia (Glerisidia maculata) yang hidup dipantai. dilakukan penelitian. Metode penelitian yang digunakan adalah metode survey sebagai penelitian paradigma naturalistik dengan prinsip yang digunakan yakni fenomenologi. Fenomena yang diamati adalah pertumbuhan dan potensi pemanfaatan tanaman untuk kehidupan manusia terutama sebagai sumber hijauan pakan. Hasil penelitian memperlihatkan bahwa tanaman Sliridia (Glerisidia maculata) mampu tumbuh dengan baik di daerah pantai hingga jarak sekitar 150 meter dari garis pantai, pada tanah berpasir, tahan kering, tetap menghasilkan daun yang hijau, berbunga dan berbuah menghasilkan malai pada kondisi kemarau yang kering dan memberikan daya dukung sebagai pakan untuk sekitar 400 ekor kambing yang terdapat di Desa Tegalretno, Kecamatan Petanahan, Kabupaten Kebumen. ABSTRACTIn order to achieve sustainable prosperity of human being, some support factors are needed, one of which is the fullfilment of animal protein requirement. One of the producers of animal protein is ruminant whit its products in the forms of meat and milk. The main feedstuff for ruminant is forage that can be supplied in part, by Glyricidia (Glyrisidia maculata), Petanahan a sub-district, coastal area of Kebumen, is one of the locations where both tame or wild Glyricidia (Glyrisidia maculata) flowishers. To know the condition of Glyricidia (Glyrisidia maculata) that grow in coastal area, a study was conducted. Survey method was employed in this research as the study of naturalistic paradigm. The study use phenomenology as its principts. The observed phenomena were the Glyricidia (Glyrisidia maculata) growth ang its utilization potential for human life. The results of this research showed that Glyricidia (Glyrisidia maculata) able to grow on coastal area up to the distance of 150 meters from the brime of coastal on the sandyland, it withsands drought. The leaves of Glyricidias (Glyrisidia maculata) are still green, the plants bear flowers and peas during dry seasons, and they can supports the life of 400 adult goats that lived in village of Tegalretno, Petanahan sub-district, the district of Kebumen.