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Moringa leaf (Moringaoleifera) is a plant that grows in Indonesia with high nutritional content. This plant has biological activity as antidiabetic, blood pressure-lowering, and antioxidant. This study analyzes the nutritional content of Moringa leaf extract, especially amino acids, minerals and determines its antioxidant. Analysis of total amino acid content has been carried out by HPLC (High performance liquid chromatography) method, minerals with ICP-OES (Inductive coupled plasma-optical emission spectroscopy) and antioxidant activity by DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The analysis of Moringa leaf extract has obtained 15 kinds of amino acids, namely: threonine (9403.09), lysine (11694.16), leucine (18087.41), isoleucine (9321.59), phenylalanine (17236.01), valine (11183.48), methionine (5684.68), tryptophan (2577.82) while non-essential amino acids include histidine (9965.39), proline (10068.07), tyrosine (8641.63), aspartate acid (16585.76), glycine (13027.13), arginine (13123.94), alanine (14474.52), glutamate acid (30106.87), serine (10055.98), cysteine (470.37) in ppm. The analysis of minerals has obtained 13 essential minerals included Al, Ca, Co, Cr, Cu, Fe, K, Mg, Mn, Ni, P, Se, Zn. The highest mineral is shown for selenium (1097.84 ppm) and chrome (919.99 ppm) at the second highest order. Sodium (68.83 ppm) and magnesium (60.84 ppm) is found as minerals with low concentrations. The antioxidant test has shown weakness activity with an average IC50 value of 9.901 μg/mL, using ascorbic acid as a positive control with IC50 values of 196.892 μg/mL. The analysis results of amino acids, minerals, and antioxidant activity show that Moringa leaves are very potential for human nutrients.

Moringa (Moringa oleifera) and Celery (Apium graveolens L) are vegetables and medicinal plants that have excellent benefits for health. The content of secondary metabolites that are very diverse in these two plants has the potential to be developed in other fields. This study aimed to determine the content of secondary metabolites and antioxidant activity of ethanol extract of moringa and celery leaves. The phytochemical analysis using the qualitative parameters of flavonoids, alkaloids, tannins, saponins, phenols, and steroids, and antioxidant activity was determined by the DPPH method. The results showed that moringa leaves contained considerable flavanoid, saponin, tannin, and alkaloid compounds while celery leaves contained fewer flavonoid, saponins, tannins, and alkaloids. Results of the antioxidant activity assay showed that moringa leaf extract had the highest activity with an IC50 value of 248.85 μg/mL while the formulation of moringa-celery (1: 1) had the lowest activity with an IC50 value of 1451.86 μg/mL. These results showed that the antioxidant activity of moringa leaf extract had better antioxidant activity than the moringa-celery extract formulation.

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