Background: Lipid Abnormalities are major risk factors for coronary artery diseases. Prevalence of dyslipidemia is high in India shown by various studies due to lifestyle changes. This study was conducted to estimate the prevalence of dyslipidemia and its association with body fat percentage, body mass index, diabetes, tobacco use and hypertension.Methods: A cross-sectional study was conducted among 300 male employees from a packaging and binding industry in Maharashtra. Behavioural data, anthropometric measurements and blood collection were conducted by trained staff using standard instruments.Results: Majority of the participants were in the age group between 30-60 years. Overall prevalence of dyslipidemia was 50.7% among study subjects.15.3%, 27% 62%, 23% and 50.7% of participants had high serum cholesterol, high triglycerides, low high density lipoprotein (HDL), high low density lipoprotein (LDL) and high cholesterol/HDL ratio respectively. Logistic regression analysis showed abnormal cholesterol level was associated with high body fat percentage and hypertension. Abnormal triglyceride level was associated with high blood sugar and hypertension. High LDL level was associated only with hypertension. Abnormal HDL level and high cholesterol/HDL ratio was strongly associated with age (>40 years).Conclusions: The prevalence of dyslipidemia was high among male industrial workers in India. Screening programs should be conducted at work places for early detection of dyslipidemia. Information, education and communication (IEC) programs based on lifestyle modification like healthy diet habits, regular physical activity for weight control and tobacco cessation need to be implemented at work places.