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Flowerhorn fish (Cichlasoma x Paraneetroplus x Amphilophus) are dangerous to the local ecosystem of Indonesian waters. It is feared that the existence of flowerhorn fish in local waters will become the dominant predator. Based on this study, it is reported that catches of flowerhorn fish in the Sempor Reservoir, Kebumen Regency, Central Java have the potential to become invasive predatory fish. The research method uses the desk study method and field observations by informants. The flowerhorn fish caught in the Sempor Reservoir is a record for efforts to protect the natural ecosystem of the reservoir from invasion by foreign predatory fish. The existence of flowerhorn fish in the Sempor Reservoir must be monitored, mapped and regulated with strict rules so that the balance of the Sempor Reservoir ecosystem is maintained from foreign predatory fish.

The objective of this research is to find out the characteristic of each test item with NOS-based instruments which is analysed based on validity and reliability level using Rasch model (RM). A test using NOS-based instruments was used to collect the data. This research is categorized as descriptive with quantitative using statistic from RM through QUEST program. There are 104 students participated as the subject of this research. The result, which is based on validity of each test item using RM analysis, shows that 25 test items are considered fit or accepted. Based on the estimate of item reliability, NOS-based instrument has reliability coefficiency at 0.96. Based on difficulty items 2 test items declared not good, namely test item number 19 and item test number 4. Based on the analysis according to Classic Test Theory of RM, the NOS instrument can be used to measure senior high school students' science literacy on Newton Law.