Introduction: Chronic Periodontitis is an infectious disease resulting in inflammation within the supporting tissues of the teeth leading to progressive attachment and bone loss. Biochemical mediators in oral fluids like saliva and gingival crevicular fluid (GCF) are highly beneficial in the determination of current periodontal status. Aim & objective: A comparative evaluation of GCF and salivary sclerostin levels in chronic periodontitis patients with or without type 2 diabetes mellitus Methodology: A total of 36 subjects in the age group of 30-60 years were categorised into three groups. Group I (Healthy individuals) Group II (chronic periodontitis patients) and Group III (chronic periodontitis patients with type 2 diabetes mellitus). During the first visit the clinical parameters like Plaque index, Gingival index, Probing pocket depth and Clinical attachment level were recorded. On the subsequent day GCF and saliva samples were collected and subjected for laboratory analysis using ELISA kit. Results were tabulated and subjected to statistical analysis. Results & Discussion: The differences of Sclerostin levels in Saliva between the groups were not statistically significant (P = 0.089). The differences of Sclerostin levels in GCF between the groups were highly statistically significant (P =0.000). Conclusion: Sclerostin levels are a promising diagnostic and prognostic biomarker in periodontal diseases.