A new species, Amblydromalus amazonicus n. sp. is described and illustrated based on adults of both sexes. Individuals were found in association with plants in natural vegetation (Amazon Forest), on uncultivated plants in guarana crops, and on exotic fruit (residential area), in states of Amazonas and Mato Grosso, Brazil. The type material of A. akiri Nuvoloni, Lofego, Rezende and Feres was re-examined and the number of setae as well as the chaetotaxy of genu III is corrected. Notes on the occurrence of Amblydromalus in South America and a key to species of genus for this subcontinent is provided.